Affordable Access

Unusual stability of a multiply nicked form of Plasmodium falciparum triosephosphate isomerase

Elsevier B.V.
Publication Date
  • Molecular Biophysics Unit
  • Biology
  • Chemistry
  • Medicine


The limited proteolytic cleavage of proteins can result in distinct polypeptides that remain noncovalently associated so that the structural and biochemical properties of the ‘nicked’ protein are virtually indistinguishable from those of the native protein. The remarkable observation that rabbit muscle triosephosphate isomerase (TIM) can be multiply nicked by subtilisin and efficiently religated in the presence of an organic solvent formed the stimulus for our study on a homologous system, Plasmodium falciparum triosephosphate isomerase (PfTIM).

There are no comments yet on this publication. Be the first to share your thoughts.