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Targeted imaging of colorectal dysplasia in living mice with fluorescence microendoscopy

Authors
Journal
Biomedical Optics Express
2156-7085
Publisher
The Optical Society
Publication Date
Volume
2
Issue
4
Identifiers
DOI: 10.1364/boe.2.000981
Keywords
  • Endoscopes
  • Catheters And Micro-Optics
Disciplines
  • Engineering

Abstract

We validate specific binding activity of a fluorescence-labeled peptide to colorectal dysplasia in living mice using a miniature, flexible, fiber microendoscope that passes through the instrument channel of an endoscope. The microendoscope delivers excitation light at 473 nm through a fiber-optic bundle with outer diameter of 680 µm to collect en face images at 10 Hz with 4 µm lateral resolution. We applied the FITC-labeled peptide QPIHPNNM topically to colonic mucosa in genetically engineered mice that spontaneously develop adenomas. More than two-fold greater fluorescence intensity was measured from adenomas compared to adjacent normal-appearing mucosa. Images of adenomas showed irregular morphology characteristic of dysplasia.

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