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Transcriptome dysregulation and epigenome signature responses in hepatocytes upon hepatitis B virus infection and therapy

Authors
Journal
Epigenetics & Chromatin
1756-8935
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Identifiers
DOI: 10.1186/1756-8935-6-s1-p67
Keywords
  • Poster Presentation
Disciplines
  • Biology
  • Medicine

Abstract

Transcriptome dysregulation and epigenome signature responses in hepatocytes upon hepatitis B virus infection and therapy POSTER PRESENTATION Open Access Transcriptome dysregulation and epigenome signature responses in hepatocytes upon hepatitis B virus infection and therapy Andreas Klein, Lisa Wiluhn, Theodor Winkler, Timo Deba, Valerie Orth, Kai Hensel, Andreas Jenke, Stefan Wirth, Jan Postberg* From Epigenetics and Chromatin: Interactions and processes Boston, MA, USA. 11-13 March 2013 Background Chronic infection with hepatitis B virus (HBV) induces transregulation of the host cell gene expression and even- tually malignant transformation. HBV X protein acts as a transactivator of cellular promoters leading to upregula- tion of DNA methyltransferases and alterations of DNA methylation patterns. Aim of the study To contribute to our understanding of DNA and chro- matin-modifying mechanisms modulated by HBV, which are responsible for the transregulation of host cells for virus particle production and malignant transformation of hepatocytes. We further study the effect of lamivudine treatment or an antiviral vector-based RNAi strategy tar- geting HBx on the establishment and maintenance of DNA methylation patterns. Our research not only aims to uncover epigenomic plasticity modulated by HBV. We also intend to contribute to the open problem, whether therapies lead to the restoration of regular epigenomic signatures, or whether hepatocytes persist in a deregu- lated epigenetic ‘memory’ state of HBV infection thus still carrying the risk for malignant transformation. Methods Therefore we analyze expression profiles by qRT PCR arrays. DNA methylation as well as chromatin signatures of genes up- or downregulated upon HBV infection are studied by MeDIP or ChIP respectively, in murine cell lines infected with HBV. Results We identified several transregulated genes and monitored DNA methylation and chromatin signatures at selected loci within promoters. Moreover we observed that t

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