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Composition and pH changes during freezing of solutions containing calcium and magnesium phosphate

Authors
Journal
Cryobiology
0011-2240
Publisher
Elsevier
Publication Date
Volume
6
Issue
1
Identifiers
DOI: 10.1016/s0011-2240(69)80003-9
Disciplines
  • Biology

Abstract

Summary Freezing tests with solutions of calcium and magnesium phosphate, with or without potassium phosphate, citrate, and chloride, showed that alkaline calcium and magnesium phosphate precipitated early during freezing when ice formation markedly increased their concentration. This precipitation caused a decrease in pH. The amounts of precipitating calcium and magnesium phosphate and the consequent pH shifts depended on initial pH and the type and concentration of other salts present. The data obtained extend earlier results on phase relations and pH changes in buffer-salt solutions during freezing and are useful in understanding pH and composition changes in the unfrozen phase of frozen biological materials.

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