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Heparin, histamine, and serotonin in normal and malignant mast cells, and nanogram determination of serotonin

Authors
Journal
Biochemical Pharmacology
0006-2952
Publisher
Elsevier
Publication Date
Volume
16
Issue
3
Identifiers
DOI: 10.1016/0006-2952(67)90102-5
Disciplines
  • Medicine

Abstract

Abstract The quantities of heparin, histamine, and 5-hydroxytryptamine in the same populations of normal rat mast cells from peritoneal washings, and of malignant (ascites tumor, line P-815) mouse mast cells, were determined per 10 6 cells. Although the normal have about twice the dry weight of the malignant cells, the heparin, histamine, and 5-HT in the former were about 20, 260, and 9 times, respectively, the quantities in the latter. Whereas the number of basic groups of the histamine plus 5-HT exceeds the number of acid groups of the heparin in the normal cells, the reverse is true for the malignant cells. The fluorometric determination of 5-HT was adapted to measurement in the range, 0.005-0.125 μg.

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