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Une methode aisee : Le tube capillaire. Prélèvement de la flore anaérobie dans les maladies parodontales

Médecine et Maladies Infectieuses
Publication Date
DOI: 10.1016/s0399-077x(05)80086-1
  • Cinquieme Partie Anaerobies Et Pathologie Bucco-Dentaire
  • Medicine


Summary The method presented here is suitable for the recuperation of total crevicular and plaque flora. The organisms of the subgingival plaque are suggested to provide a suitable anerobic environment for the proliferation of motile bacteria in crevicular fluid, as the latter appear to be increasingly important in the pathogenesis of periodontal diseases. Samples were collected using a capillary tube (Microcaps, 1 μl); after superficial cleaning of the gingival margin with a sterile toothpick, the capillary tube is then iserted into the gingival pocket up to its fulldepth, while inserting the tube; the tip of the glass rod was used to scrape the coaggregated bacteria. The microorganisms of the subgingival plaque and crevicular fluid were aspirated together by capillarity. The tubes are then sealed with wax and sent to the laboratory. The capillary tube is then crushed with a glass bead in a sterile tube containing 3 drops of sterile water. The crushed sample is spread on different media for anaerobic and microaerophilic bacteria. Among the potential pathogens, this technique permits to recover Fusobacterium nucleatum with a frequency of more than 90%, Bacteroides intermedius 80%, Centipeda periodontii 59%, Treponema sp. 55%, Selenomonas sp. 55%, Bacteroides gracilis 45%, Eikenella corrodens 45%, Wolinella recta 40%, Capnocytophaga sp. 35%, Bacteroides gingivalis 25%, Eubacterium yurii 25% and Actinobacillus actinomycetemcomitans 5%. The usual means of sampling subgingival plaques are by scraping with a curette, cotton swab probe or micro-syringe with a blunted needle, and the material is then sent to the laboratory using various transport media. Unfortunately, these types of sampling techniques do not allow recovery of dental plaque and crevicular fluid flora together.

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