Affordable Access

MAREK’S DISEASE VIRAL ANTIBODIES DETECTION USING INDIRECT ENZYME LINKED IMMUNOSORBENT ASSAY IN DIFFERENT AVIAN SPECIES, NIGERIA

Authors
Publisher
Continental Journal of Veterinary Sciences
Publication Date
Disciplines
  • Biology
  • Medicine

Abstract

Marek's disease has been reported in both vaccinated and unvaccinated flocks worldwide. Thisstudy reported a survey of Marek's disease virus (MDV) antibodies of 151 randomly selectedsera from different avian species from FCT 59.09% (13/22), Nassarawa 40% (8/20), Kaduna45% (18/40), Bauchi 60% (6/10) and Plateau 84.75% (50/59) states of Nigeria were observed tobe positive. The seroprevalence of MDV was examined using the indirect-ELISA madeavailable in 96- round bottom wells of microtitre plate coated with MDV-infected cells fromcommercial vaccines as antigen in coated buffer. The optical density was read at 492nm usingELISA plate reader. The overall Marek's disease virus antibody present was 62.91% (95/151) ofthe screened sera while, 37.09% (56/151) were negative. On type of avian species, out of 108sera from commercially vaccinated chickens, 68.52% (74/108) were positive and out of 17locally unvaccinated chickens, 76.48% (13/17) tested positive for MD. Other sera from avianspecies screened were, 12 Turkeys, 12 Quails and 2 Ducks with the corresponding results; 5(41.67%) and 3 (25%) for Turkeys and Quails respectively while Ducks tested negative for MD.Indirect enzyme linked immunosorbent assay is sensitive and specific which can be used toscreen sera from large field samples for serological survey. KEY WORDS: Marek's disease, ELISA, seroprevalence, avian species, Nigeria

There are no comments yet on this publication. Be the first to share your thoughts.