Affordable Access

Publisher Website

Central Region of Talin Has a Unique Fold That Binds Vinculin and Actin*

Journal of Biological Chemistry
American Society for Biochemistry and Molecular Biology
Publication Date
DOI: 10.1074/jbc.m109.095455
  • Protein Structure And Folding
  • Biology


Talin is an adaptor protein that couples integrins to F-actin. Structural studies show that the N-terminal talin head contains an atypical FERM domain, whereas the N- and C-terminal parts of the talin rod include a series of α-helical bundles. However, determining the structure of the central part of the rod has proved problematic. Residues 1359–1659 are homologous to the MESDc1 gene product, and we therefore expressed this region of talin in Escherichia coli. The crystal structure shows a unique fold comprised of a 5- and 4-helix bundle. The 5-helix bundle is composed of nonsequential helices due to insertion of the 4-helix bundle into the loop at the C terminus of helix α3. The linker connecting the bundles forms a two-stranded anti-parallel β-sheet likely limiting the relative movement of the two bundles. Because the 5-helix bundle contains the N and C termini of this module, we propose that it is linked by short loops to adjacent bundles, whereas the 4-helix bundle protrudes from the rod. This suggests the 4-helix bundle has a unique role, and its pI (7.8) is higher than other rod domains. Both helical bundles contain vinculin-binding sites but that in the isolated 5-helix bundle is cryptic, whereas that in the isolated 4-helix bundle is constitutively active. In contrast, both bundles are required for actin binding. Finally, we show that the MESDc1 protein, which is predicted to have a similar fold, is a novel actin-binding protein.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Central region of talin has a unique fold that bin...

on Journal of Biological Chemistr... Sep 17, 2010

Thermodynamics of talin and talin-vinculin binding...

on Biochemical Society Transactio... February 1992

Temperature dependent specific heat capacity (Cp)...

on Biochemical Society Transactio... August 1992

The vinculin binding sites of talin and alpha-acti...

on Journal of Biological Chemistr... Mar 17, 2006
More articles like this..