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Applications of Fluorescence Correlation Spectroscopy— Particle Size Effect

Authors
Journal
Journal of Colloid and Interface Science
0021-9797
Publisher
Elsevier
Publication Date
Volume
203
Issue
1
Identifiers
DOI: 10.1006/jcis.1998.5470
Keywords
  • Fluorescence Correlation Spectroscopy
  • Fcs
  • Latex Particles
  • Rhodamine 6G
  • Monte Carlo Simulation
  • Diffusion Coefficient

Abstract

Abstract Both computer simulations and experiments have been employed to study the fluorescence correlation spectroscopy autocorrelation function of particles with size comparable to the size of the light beam. When such particles are labeled by several fluorophores the characteristic time of diffusion and the particle number in the light beam are larger than those expected from the existing theory developed for the case of a point size diffuser. The resulting effect is described by simple equations.

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