Affordable Access

Publisher Website

Methods for isolation of cell-free plasma DNA strongly affect DNA yield

Clinica Chimica Acta
Publication Date
DOI: 10.1016/j.cca.2011.07.011
  • Plasma Dna
  • Isolation Method
  • Real-Time Pcr
  • Quantification


Abstract Extracellular nucleic acids are present in plasma, serum, and other body fluids and their analysis has gained increasing attention during recent years. Because of the small quantity and highly fragmented nature of cell-free DNA in plasma and serum, a fast, efficient, and reliable isolation method is still a problem and so far there is no agreement on a standardized method. We used spin columns from commercial suppliers (QIAamp DNA Blood Midi Kit from Qiagen; NucleoSpin Kit from Macherey-Nagel; MagNA Pure isolation system from Roche Diagnostics) to isolate DNA from 44 plasma samples in parallel at laboratories in Berlin and Munich. DNA in all samples was quantified by real-time PCR on a LightCycler 480 using three different targets (GAPDH, ß-globin, ERV). The quantities of cell-free DNA for the different isolation methods and genes varied between medians of 1.6ng/mL and 28.1ng/mL. This considerable variation of absolute DNA values was mainly caused by the use of different isolation methods (p<0.0001). Comparable results were achieved by the use of the genes GAPDH and ERV while higher values were obtained by use of ß-globin. The laboratory site had only minor influence on DNA yield when manual extraction methods were used.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Quantity versus quality: optimal methods for cell-...

on Genetics in Medicine October 2006

Improvement of methods for the isolation of cell-f...

on Annals of the New York Academy... September 2006

An improved method for the isolation of free-circu...

on Annals of the New York Academy... August 2008

Optimizing the yield and utility of circulating ce...

on Clinica Chimica Acta Jan 01, 2009
More articles like this..