Affordable Access

Surface Localization of Escherichia coli 5′-Nucleotidase by Electron Microscopy

Authors
Publication Date
Source
PMC
Keywords
  • Microbial Physiology And Metabolism
Disciplines
  • Chemistry

Abstract

The 5′-nucleotidase of Escherichia coli was shown to be located at the cell wall surface by histochemical techniques utilizing the deposition of inorganic phosphate. Penetration of the 5′-nucleotidase in the periplasmic space was seen only in cells treated with ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)aminomethane (Tris). The 3′-nucleotidase of E. coli was also found to have a surface location, and periplasmic precipitation of inorganic phosphate was seen only after EDTA-Tris-sucrose exposure.

There are no comments yet on this publication. Be the first to share your thoughts.