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Specific labelling of the (Ca2++ Mg2+)-ATPase ofEscherichia coliwith 8-azido-ATP and 4-chloro-7-nitrobenzofurazan

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Bioenergetics
0005-2728
Publisher
Elsevier
Publication Date
Volume
502
Issue
2
Identifiers
DOI: 10.1016/0005-2728(78)90054-3
Disciplines
  • Biology

Abstract

Abstract 1. 8-Azido-ATP is a substrate for Escherichia coli (Ca 2+ + Mg 2+)-ATPase ( E. coli F 1). 2. Illumination of E. coli F 1 in the presence of 8-azido-ATP causes inhibition of ATPase activity. The presence of ATP during illumination prevents inhibition. 3. 8-Azido-ATP and 4-chloro-7-nitrobenzofurazan (NbfCl) bind predominantly to the α subunit of the enzyme, but also significantly to the β subunit. 4. The α subunit of E. coli F 1 seems to have some properties that in other F 1-ATPases are associated with the β subunit.

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