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Hydrogen production by the newly isolatedClostridium beijerinckiiRZF-1108

Authors
Journal
Bioresource Technology
0960-8524
Publisher
Elsevier
Publication Date
Volume
102
Issue
18
Identifiers
DOI: 10.1016/j.biortech.2011.02.086
Keywords
  • Biohydrogen Production
  • Hydrogen-Producing Bacterium
  • Clostridium Beijerinckii
  • Isolation
  • Culture Condition
Disciplines
  • Chemistry
  • Medicine

Abstract

Abstract A fermentative hydrogen-producing strain, RZF-1108, was isolated from a biohydrogen reactor, and identified as Clostridium beijerinckii on the basis of the 16S rRNA gene analysis and physiobiochemical characteristics . The effects of culture conditions on hydrogen production by C. beijerinckii RZF-1108 were investigated in batch cultures. The hydrogen production and growth of strain RZF-1108 were highly dependent on temperature, initial pH and substrate concentration. Yeast extract was a favorable nitrogen source for hydrogen production and growth of RZF-1108. Hydrogen production corresponded to cell biomass yield in different culture conditions. The maximum hydrogen evolution, yield and production rate of 2209 ml H 2/l medium, 1.97 mol H 2/mol glucose and 104.20 ml H 2/g CDW h −1 were obtained at 9 g/l of glucose, initial pH of 7.0, inoculum volume of 8% and temperature of 35 °C, respectively. These results demonstrate that C. beijerinckii can efficiently produce H 2, and is another model microorganism for biohydrogen investigations.

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