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Phenylalanine in whole blood and plasma — a candidate reference method

Authors
Journal
Journal of Chromatography B Biomedical Sciences and Applications
0378-4347
Publisher
Elsevier
Publication Date
Volume
562
Identifiers
DOI: 10.1016/0378-4347(91)80579-2

Abstract

Abstract A method for the quantification of phenylalanine in whole blood and plasma by isotope dilution gas chromatography—mass spectrometry is presented. The use of an uncommon derivative allows a simple extraction procedure and the most basic mass spectrometer. The relative standard deviation was found to be 1.3% within-batch and 2.1% between-batch under optimum conditions, and the detection limit was found to be 7 pmol injected. The ruggedness of the procedure and sample handling conditions were also examined.

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