Affordable Access

Amplification and Re-Generation of LNA-Modified Libraries

Authors
Publisher
Molecular Diversity Preservation International (M D P I)
Publication Date
Keywords
  • Locked Nucleic Acids (Lna) Confer High Thermal Stability And Nuclease Resistance To Oligonucleotides
  • The Discovery Of Polymerases That Accept Lna Triphosphates Has Led Us To Propose A Scheme For The Am
  • Such Libraries Could Be Used For In Vitro Selection Of E
  • G
  • Native Lna Aptamers
  • We Maintained An Oligonucleotide Library Encoding 40 Randomized Positions With Lna Atp
  • Gtp
  • Ctp
  • And Ttp For 7 Rounds Of ‘Mock’ In Vitro Selection In The Absence Of A Target And Analyzed The Se
  • 4 And 7
  • We Observed A Decrease In Lna-A Content From 20
  • 5% In Round 1 To 6
  • 6% In Round 7
  • This Decrease Was Accompanied By A Substantial Bias Against Successive Lna-As (Poly-Lna Adenosine Tr
  • Maintaining A Library With Lna Ttp Yielded Similar Results
  • Together
  • These Results Suggest That Dispersed Lna Monomers Are Tolerated In Our In Vitro Selection Protocol
  • And That Lna-Modified Libraries Can Be Sustained For Up To At Least Seven Selection Rounds
  • Albeit At Reduced Levels
  • This Enables The Discovery Of Native Lna Aptamers And Similar Oligonucleotide Structures

Abstract

Amplification and Re-Generation of LNA-Modified Libraries - DTU Orbit (21/04/14) Amplification and Re-Generation of LNA-Modified Libraries - DTU Orbit (21/04/14) Amplification and Re-Generation of LNA-Modified Libraries. / Doessing, Holger; Hansen, Lykke H.; Veedu, Rakesh N.; Wengel, Jesper; Vester, Birte. In: Molecules, Vol. 17, No. 11, 2012, p. 13087. Publication: Research - peer-review › Journal article – Annual report year: 2012

There are no comments yet on this publication. Be the first to share your thoughts.