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Steroidal compounds from in vitro regenerated shoots ofDatura metel

Authors
Journal
Fitoterapia
0367-326X
Publisher
Elsevier
Publication Date
Volume
74
Identifiers
DOI: 10.1016/s0367-326x(02)00310-6
Keywords
  • Datura Metel
  • Regeneration

Abstract

Abstract Regeneration of adventitious shoots from young leaves of Datura metel is described. Shoot buds developed on MS medium with 2 mg/l and elongated on hormone-free solid basal medium. The microshoots failed to produce alkaloids, but a number of steroidal compounds were detected. The C 28 sterol 3 β,24 ξ-dihydroxy-ergosta-5,25-dienolide, regarded as the precursor of withanolides and related steroidal lactones, was identified. The withanolide 12-deoxywithastramonolide was also detected.

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