Affordable Access

Publisher Website

Cryofibrinogen in the plasma of patients with skin ulcerative lesions on the legs: A complex of fibrinogen and cold insoluble globulin

Authors
Journal
Thrombosis Research
0049-3848
Publisher
Elsevier
Publication Date
Volume
9
Issue
6
Identifiers
DOI: 10.1016/0049-3848(76)90102-x
Disciplines
  • Biology

Abstract

Abstract Cryofibrinogen was separated from the plasma of four patients with skin ulcers on the legs. It was identified as a complex of fibrinogen and the cold insoluble globulin based on its sedimentation coefficients, electrophoretic mobilities, thrombin-clottability and amino acid compositions. It formed a solid gel with fibrin strands in a short time at 4°C and readily redissolved at 37°C. The fibrinogen in the fraction was not significantly affected by proteolytic enzymes since its subunits were found to be well retained when examined by SDS polyacrylamide gel electrophoresis. The heat-defibrination at 56°C for 3 min resulted in a loss of gel forming capacity, however, the reconsitution with an equivalent amount of purified human fibrinogen apparently recovered it to a certain extent, but not to the initial level. The sedimentation coefficient of the non-fibrinogen fraction fell between 11 S and 13 S and its estimated molecular weight was 4.4 × 10 5. The amino acid compositions were found to be consistent with those recently reported on the cold insoluble globulin in the normal plasma.

There are no comments yet on this publication. Be the first to share your thoughts.