Affordable Access

Publisher Website

Microcalorimetric studies on the adsorption of DNA by soil colloidal particles

Authors
Journal
Colloids and Surfaces B Biointerfaces
0927-7765
Publisher
Elsevier
Publication Date
Volume
49
Issue
1
Identifiers
DOI: 10.1016/j.colsurfb.2006.02.011
Keywords
  • Microcalorimetry
  • Dna Adsorption
  • Enthalpy Change
  • Permanent-Charge
  • Variable-Charge
  • Soil Colloids
Disciplines
  • Physics

Abstract

Abstract This study applied TAM air isothermal calorimeter to measure the adsorption enthalpies of DNA on eight colloidal fractions from permanent-charge and variable-charge soils. The adsorption of DNA on soil colloids was also examined by equilibrium adsorption analysis. The data evaluated from isotherms fitted by Langmuirean model revealed that the affinity of DNA for variable-charge soil colloids was higher than that for permanent-charge soil colloids. More tightly bound DNA molecules were observed on coarse clays and inorganic clays than on fine clays and organic clays, respectively. The adsorption enthalpies of DNA on permanent-charge soil colloids were higher than those on variable-charge soil colloids. DNA adsorption on organic clays is endothermic, whereas that on inorganic clays is exothermic. Dehydration and electrostatic repulsion were considered to cause the higher adsorption enthalpies of DNA with organic clays, while hydrogen bonding, ligand exchange and electrostatic attraction result in the lower DNA adsorption enthalpies on inorganic clays. The thermodynamic parameters presented in this study have important implication for providing further insight into mechanisms of the adsorption of DNA on soil particles.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Microcalorimetric studies on the adsorption of DNA...

on Colloids and Surfaces B Bioint... Apr 15, 2006

[Characteristics of DNA adsorption on different si...

on Ying yong sheng tai xue bao =... March 2013

Adsorption of DNA on clay minerals and various col...

on Soil Biology and Biochemistry Jan 01, 2006

Interactions of DNA with clay minerals and soil co...

on Environmental science & techno... May 01, 2006
More articles like this..