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Studies on the anaerobic degradation of crystalline cellulose byClostridium thermocellumusing a new assay

Authors
Journal
FEMS Microbiology Letters
0378-1097
Publisher
Oxford University Press
Publication Date
Volume
72
Identifiers
DOI: 10.1016/0378-1097(90)90372-w
Keywords
  • Clostridium Thermocellum
  • Cellulase Assay
  • Cellulolytic Complex
  • Michaelis-Menten Kinetics
Disciplines
  • Biology

Abstract

Abstract The anaerobic degradation of microcrystalline cellulose by thermostable cellulolytic enzyme complexes from Clostridium thermocellum JW20 (ATCC 31449) was monitored. For quantitative investigations as enzyme-coupled spectrophotometric assay has been developed. The assay allows for the evaluation of the release of cellubiose-/glucose-units from native cellulose. Kinetic studies revealed that the anaerobic breakdown of crystalline cellulose (CC) at 60°C follows Michaelis-Menten kinetics K m CC values have been determined for different aggregation states of the cellulolytic complex. The presented assay seems well suited to screen for CC-degrading enzymes of various sources, and to further explore the mechanism of CC-breakdown.

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