Affordable Access

Publisher Website

New derivatization method for carboxylic acids in aqueous solution for analysis by capillary electrophoresis and laser-induced fluorescence detection

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
836
Issue
2
Identifiers
DOI: 10.1016/s0021-9673(99)00012-6
Keywords
  • Aminofluorescein
  • Carboxylic Acids
  • Dicyclohexylcarbodiimide

Abstract

Abstract A new derivatization method for carboxylic acids in aqueous solution for analysis with capillary electrophoresis and laser-induced fluorescence detection was developed. The derivatization is based on a phase-transfer reaction with 4-aminofluorescein as fluorophore and dicyclohexylcarbodiimide as activating agent. This reaction procedure includes an enrichment factor of the analyte up to 10-fold. The limits of detection of carboxylic acids are in the range of 3–150 nmol/l. An advantage of this system is the indifference against inorganic matrices like carbonate, chloride, etc., up to the mmol/l range. Using this technique for the first time, the measurement of diurnal profiles of carboxylic acids (C 5–C 9) in ambient air with a time resolution of 1 h is possible.

There are no comments yet on this publication. Be the first to share your thoughts.