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Separate regulatory control of apical and basolateral Na+/H+exchange in renal epithelial cells

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
165
Issue
2
Identifiers
DOI: 10.1016/s0006-291x(89)80041-5

Abstract

Epithelial layers of LLC-PK 1/PKE 20 cells, a renal epithelial cell line which expresses Na +/H + exchange activities in the apical as well as basolateral membrane domains, are examined in the single cell mode by microspectrofluorometry. We provide evidence that basolateral Na +/H + exchange is more sensitive to amiloride inhibition than is apical Na +/H + exchange. Furthermore, we demonstrate that the two exchange activities differ in their regulatory control: kinase A activation (forskolin, 8-Br-cAMP) leads to inhibition of both exchange activities, whereas kinase C activation (phorbol ester) stimulates basolateral and inhibits apical Na +/H + exchange. Thus, renal epithelial cells may contain two Na +/H + exchange activities: an apical (“epithelial”) and basolateral (“housekeeping”) which may serve different cellular functions and are under separate regulatory controls.

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