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The Anatomy of the cell envelope of a marine vibrio examined by freeze-etching

Authors
Publisher
University of Florida
Publication Date
Keywords
  • Cytology -- Research ( Lcsh )
  • Bacterial Cell Walls ( Lcsh )
  • Microbiology Thesis Ph. D
  • Dissertations
  • Academic -- Microbiology

Abstract

The anatomy of the cell envelope of a marine vibrio examined by freeze-etching THE ANATOMY OF THE CELL ENVELOPE OF A MARINE VIBRIO EXAMINED BY FREEZE-ETCH I NG By JACK T. CRAWFORD A DISSERTATION PRESENTED TO THE GRADUATE COUNCIL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY UNIVERSITY OF FLORIDA 1973 ACKNOWLEDGMENTS I wish to express my appreciation to the following people whose efforts made this study possible. I wish to thank Dr. Max E. Tyler for his guidance, encouragement, and patience during the course of this study. I wish to thank Dr. Henry C. Aldrich for his considerable efforts in my training in all aspects of electron microscopy, for the use of the facilities of the Biological Ul trastructure Laboratory, and for his encouragement and friendship. I wish to thank Dr. Arnold S. Bleiv/eis for serving on my advisory convnittee and for the use of his facilities in preparing the amino acid anal yses. I wish to thank Dr. Daniel Billen for serving on my advisory commi ttee. I wish to especially thank my wife, Kay, for her unfailing under- standing and patience. I I ACKNOWLEDGHENTS , . . LrST OF TABLES . . , LIST OF FIGURES , . , KEY TO SYMBOLS . . . ABSTRACT INTRODUCTION . . . , MATERIALS AND METHODS RESULTS DISCUSSION LITERATURE CITED . . BIOGRAPHICAL SKETCH . TABLE OF CONTENTS Page ' ' ° ii iv * ' V ix • ' X 1 13 25 89 93 105 1 1 1 LIST OF TABLES Table ]. 2. Amino acid analysis of purified peptidoglycan Determination of the specificity of antibody label 1 i ng Page 29 78 IV LIST OF FIGURES Figure Page 1. Longitudinal and cross sections of cells fixed in gl utaraldehyde and OsOr 27 2. Cross section of a cell shov;ing the double- track appearance of the membranes 27 3. Cross section of a cell showing an extra layer inside the cytoplasmic membrane 27 k. Purified trypsi n-treated pe

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