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Gas-phase protein sequencing: gas flow control by positive displacement

Authors
Journal
Journal of Biochemical and Biophysical Methods
0165-022X
Publisher
Elsevier
Publication Date
Volume
17
Issue
3
Identifiers
DOI: 10.1016/0165-022x(88)90008-5
Keywords
  • Protein
  • Peptide
  • Amino Acid
  • Edman Degradation
  • Sequence
Disciplines
  • Biology
  • Chemistry

Abstract

Abstract A modification of an automatic gas-phase protein/peptide sequencing apparatus is described; this eliminates the effect of the sample on cell gas flow rates. Consistent sequencing chemistry is achieved, yielding data from material that is intractable using standard equipment.

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