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Phylogeny of lymphocyte heterogeneity. IV. Evidence for T-like and B-like cells in reptiles

Authors
Journal
Developmental & Comparative Immunology
0145-305X
Publisher
Elsevier
Publication Date
Volume
3
Identifiers
DOI: 10.1016/s0145-305x(79)80042-7

Abstract

Abstract Peripheral blood lymphocytes from the Florida alligator were separated into at least two subpopulations based upon surface markers and mitogen responsiveness. Fractionation on glasswool columns yielded two subpopulations, a nonadherent one responsive in vitro to PHA and Con A but not to LPS, and an adherent one responsive to LPS (and to PHA). Depletion of cells bearing surface immunoglobulin determinants (by complement-mediated cytotoxicity and by anti-immunoglobulin affinity columns) ablated the responsiveness to LPS (and PWM) but not to PHA. LPS, but not PHA, induced a subpopulation of alligator lymphocytes to differentiate into immunoglobulin producing cells. These data, and other related studies, indicate the Florida alligator likely has T and B cells akin to those of mammals and birds.

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