Affordable Access

Publisher Website

Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens

Authors
Journal
Clinical Immunology
1521-6616
Publisher
Elsevier
Publication Date
Volume
127
Issue
3
Identifiers
DOI: 10.1016/j.clim.2008.01.018
Keywords
  • Autoantibodies
  • Autoantigens
  • Gad65
  • Ia-2
  • Protein Tyrosine Phosphatase
  • Proteome
  • Secretory Vesicles
  • Type 1 Diabetes
Disciplines
  • Biology
  • Medicine

Abstract

Abstract The four major autoantigens (IA-2, IA-2β, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with secretory vesicles, were prepared by in vitro transcription/translation and screened for autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated proteins, VAMP2 and NPY, were identified as new minor autoantigens with 21% and 9%, respectively, of 200 type 1 diabetes sera reacting positively. These findings add support to the hypothesis that secretory vesicle-associated proteins are particularly important, but not the exclusive, targets of the autoimmune response in type 1 diabetes. Selective screening of the human proteome offers a useful approach for identifying new autoantigens in autoimmune diseases.

There are no comments yet on this publication. Be the first to share your thoughts.