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Functional expression of a plant plasma membrane transporter inXenopusoocytes

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
302
Issue
2
Identifiers
DOI: 10.1016/0014-5793(92)80431-f
Keywords
  • Arabidopsis Thaliana
  • Hexose Transporter
  • Proton Symporter
  • Heterologous Expression
  • Xenopusoocyte

Abstract

Abstract A full-length cDNA clone for the H +/hexose co-transporter (STP1) from Arabidopsis thaliana has been transcribed in vitro and the mRNA injected into Xenopus oocytes. Under optimized conditions, oocytes injected with the STPI mRNA accumulated 3- O-[methyl- 14C]glucose at rates of more than a 1000-fold greater than water-injected control oocytes. A hexose-elicited depolarization of the oocyte membrane potential was demonstrated, and uptake was shown to be stimulated by low external pH, confirming the activity of a H +/hexose co-transport system. This is the first example of the functional expression of a plant membrane transporter in oocytes.

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