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Trimethyltin inhibits the chemotaxis ofDictyostelium discoideumamoebae

Authors
Journal
European Journal of Protistology
0932-4739
Publisher
Elsevier
Publication Date
Volume
37
Issue
3
Identifiers
DOI: 10.1078/0932-4739-00834
Keywords
  • Cell Migration
  • Chemotaxis
  • Dictyostelium Discoideum
  • Trimethyltin
  • Microtubules

Abstract

The effect of trimethyltin (TMT) on the chemotaxis of Dictyostelium discoideum amoebae in developing concentration gradients of folic acid (FA), cAMP, Ca 2+, and Mg 2+ has been studied with quantitative, computer-assisted methods. A “U” shaped pocket-like chamber, permitting the monitoring of the locomotion of individual cells, was used to observe the chemotactic response of amoebae. We found that TMT slightly affected the motile activity of cells but completely inhibited the chemotactic response of Dictyostelium discoideum cells towards all the tested chemoattractants. Moreover, the treatment of Dictyostelium amoebae with TMT caused the disruption of microtubules, suggesting that they have a role in the polarization and directional movement of these cells.

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