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Dye-sensitized photo-oxidation as a tool for mapping some amino acid residues in proteins

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
38
Issue
1
Identifiers
DOI: 10.1016/0006-291x(70)91077-6
Disciplines
  • Biology

Abstract

Abstract The irradiation of proteins containing a sensitizer covalently bound in known positions of the molecule (e.g. 41-DNP-RNase A) results in the selective modification of the amino acid residues which are adjacent to the sensitizer within the molecule. In this way, the location of the modified side chains in the three-dimensional network of the protein can be deduced.

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