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Experimental infection of murine and human macrophages withCystoisospora belli

Authors
Journal
Acta Tropica
0001-706X
Publisher
Elsevier
Publication Date
Volume
111
Issue
2
Identifiers
DOI: 10.1016/j.actatropica.2009.04.010
Keywords
  • Cystoisospora Belli
  • Macrophage Culture
  • Isospora Belli
  • Unizoic Cysts

Abstract

Abstract Extraintestinal cystoisosporosis by Cystoisospora belli has already been reported in HIV/AIDS patients, generally involving preferential invasion of mesenteric and trachaeobronchial lymph nodes, liver and spleen by unizoic cysts of this parasite, which may infect macrophages. To test this hypothesis, murine and human macrophages were exposed to sporozoites of C. belli and cultures were observed daily after contact with these cells. The parasites penetrated and multiplied by endodyogeny in both cell types and inserted themselves inside perinuclear vacuoles. After 48 h, extracellular parasites were removed from macrophage cultures and incubated in Monkey Kidney Rhesus cells (MK2) where there was intense multiplication. This is the first report of infection of macrophages by this parasite, which supports the hypothesis that these could act as C. belli host cells in extraintestinal sites.

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