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Investigation of metallodrug–protein interactions by size-exclusion chromatography coupled with inductively coupled plasma mass spectrometry (ICP-MS)

Authors
Publisher
Elsevier B.V.
Publication Date
Volume
387
Issue
2
Identifiers
DOI: 10.1016/s0003-2670(99)00074-4
Keywords
  • Platinum
  • Ruthenium
  • Antitumor Drugs
  • Speciation
  • Sec
  • Icp-Ms
  • Proteins
Disciplines
  • Biology

Abstract

Abstract The coupling of size-exclusion HPLC with ICP-MS was developed for the studies of the kinetics of metallodrug binding to human serum proteins. Two platinum- and three ruthenium-based drugs were investigated. Various SEC columns (of different lengths and with different packings) were compared for the separation of the protein-bound and unbound fractions of a metallodrug prior to on-line detection of the metal (Ru or Pt). The approach developed offers considerable advantages over the methods based on ultrafiltration followed by the off-line metal determination in terms of speed, simplicity, precision and selectivity regarding the molecular weight of the complexes involved.

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