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Development and validation of a high-performance liquid chromatography method for the determination of cold relief ingredients in chewing gum

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
775
Identifiers
DOI: 10.1016/s0021-9673(97)00303-8
Keywords
  • Paracetamol
  • Pseudoephedrine
  • Chlorpheniramine

Abstract

Abstract An isocratic high-performance liquid chromatography (HPLC) method for the simultaneous determination of the active compounds paracetamol, pseudoephedrine and chlorpheniramine in chewing gum samples has been developed and validated. The method required a simple liquid–liquid extraction using n-hexane and a mixture of water–acetonitrile prior to HPLC analysis. The chromatographic separation was achieved with an aqueous solution containing hexylamine (pH 3)–acetonitrile as the mobile phase, a Spherisorb C18 column and UV detection at 220 nm. As an application, the proposed method has been used to evaluate the release into saliva of these compounds from samples under a controlled human chewing process.

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