This book is both an introductory text and a lab manual for a course in recombinant DNA technology. The topics of the eight chapters of text include background, basic and advanced tools and techniques, gene regulation in development, and applications. Topics of some of the ten highly detailed lab exercises are bacterial culture techniques, DNA restriction analysis, recombination of antibiotic-resistant genes, and replica plating in identification of bacterial populations. Appendices include recipes for media and solutions. The text closes with a bibliography and a combination glossary/index.