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Monoclonal anti-actin antibody recognizes a surface molecule on normal and transformed human B lymphocytes: Expression varies with phase of cell cycle

Authors
Journal
Cellular Immunology
0008-8749
Publisher
Elsevier
Publication Date
Volume
98
Issue
2
Identifiers
DOI: 10.1016/0008-8749(86)90296-0
Disciplines
  • Biology

Abstract

Abstract A monoclonal anti-actin antibody, 2C9, was used to study the distribution of an actin-like cell-surface antigen (hereafter termed actin) on a lymphoblastoid cell line LA350 and on human peripheral blood lymphocytes. It was determined that 8–40% of LA350 cells and 3–15% of peripheral blood lymphocytes from healthy donors stain specifically with 2C9, almost exclusively on IgM-positive cells. Treatment of cells with 2C9 prior to incubation caused cell-surface actin to first patch and then to cap. Treatment of cells with nonspecific protease caused a loss of surface actin, with reexpression of the marker after 8–12 hr. The expression of LA350 surface actin also increased with DNA synthesis and was demonstrated to be maximal during late G 1/early S phase. Thus, this antigen may be a sensitive marker for activated lymphocytes. These studies contribute to our understanding of the expression and distribution of actin-like membrane proteins that may participate in regulatory signals mediated by anti-actin antibody.

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