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The incorporation ofO6-methyldeoxyguanosine monophosphate andO4-methyldeoxythymidine monophosphate into polynucleotide templates leads to errors during subsequent in vitro DNA synthesis

Authors
Journal
Chemico-Biological Interactions
0009-2797
Publisher
Elsevier
Publication Date
Volume
56
Identifiers
DOI: 10.1016/0009-2797(85)90017-1
Keywords
  • Dna Synthesis
  • Miscoding
  • Mechanisms Of Mutagenesis
  • Promutagenicity

Abstract

Abstract O 4-MedTMP and O 6-MedGMP may be incorporated from their 5′-triphosphates into polynucleotides by DNA polymerases. Once incorporated O 6-MedGMP leads to errors during further cycles of DNA synthesis with a mutagenic efficiency of 0.3–0.7, the value depending upon the relative dNTP concentrations in the assay. In contrast O 4-MedTMP leads to very few errors following its incorporation into DNA. The results demonstrate that O 6-MedGMP, once incorporated into DNA, should be destined to give rise to DNA base changes during the second round of DNA synthesis.

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