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Evaluation of the genetic diversity of a set of parents of Eucalyptus spp. by using microsatellite markers to direct matings

Authors
Journal
BMC Proceedings
1753-6561
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
5
Identifiers
DOI: 10.1186/1753-6561-5-s7-p55
Keywords
  • Poster Presentation
Disciplines
  • Biology
  • Design

Abstract

Evaluation of the genetic diversity of a set of parents of Eucalyptus spp. by using microsatellite markers to direct matings POSTER PRESENTATION Open Access Evaluation of the genetic diversity of a set of parents of Eucalyptus spp. by using microsatellite markers to direct matings Lucas Santos1*, Eullaysa Sabóia1, Douglas Almeida2, Jupiter Abad3, Alexandre Missiaggia3, Norma Pereira1, Dário Ahnert1, Fernanda Gaiotto1, Ronan Corrêa1 From IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and Delivery Arraial d’Ajuda, Bahia, Brazil. 26 June - 2 July 2011 Background To obtain genetically superior cultivars in a breeding program, methods and procedures are necessary to allow the identification of selected individuals over sev- eral cycles of selection while at the same time maintain broad genetic base of the breeding populations. This is crucial to guarantee continuous genetic gains along the program. The establishment of efficient breeding strate- gies depends on methods and analytical tools. The assessment of genetic diversity with molecular markers of parents used in mating designs could aid optimizing the recombination phase. Microsatellites provide good information content and require small amounts of DNA and may be transferable between species of the same genus. In this study we evaluated the genetic diversity in a set of Eucalyptus parent trees and indicated those to be preferentially crossed in a recombination process to potentially maximize variation in the offspring for indi- vidual selection of clones. Materials and methods A total of 20 genotypes were selected from 44 families obtained by crossing 24 parents in different types of hybridizations of populations of Eucalyptus grandis and E.urophylla. Genomic DNA was extracted from leaves and amplified with microsatellites developed during the Genolyptus project and previously used for Eucalyptus spp[1]. The amplifications were performed using the GeneAmp PCR System 9600 (Applied Biosystems) ther- mal cyc

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