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In vitro digestion and lactase treatment influence uptake of quercetin and quercetin glucoside by the Caco-2 cell monolayer

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BioMed Central
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PMC
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  • Research

Abstract

1475-2891-4-1.fm ral ss BioMed CentNutrition Journal Open AcceResearch In vitro digestion and lactase treatment influence uptake of quercetin and quercetin glucoside by the Caco-2 cell monolayer Jeanelle Boyer1, Dan Brown1,2 and Rui Hai Liu*2,3 Address: 1Department of Animal Science, Cornell University, Ithaca, New York 14853-7201, USA, 2Institute of Comparative and Environmental Toxicology, Cornell University, Ithaca, New York 14853-7201, USA and 3Department of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853-7201, USA Email: Jeanelle Boyer - [email protected]; Dan Brown - [email protected]; Rui Hai Liu* - [email protected] * Corresponding author Abstract Background: Quercetin and quercetin glycosides are widely consumed flavonoids found in many fruits and vegetables. These compounds have a wide range of potential health benefits, and understanding the bioavailability of flavonoids from foods is becoming increasingly important. Methods: This study combined an in vitro digestion, a lactase treatment and the Caco-2 cell model to examine quercetin and quercetin glucoside uptake from shallot and apple homogenates. Results: The in vitro digestion alone significantly decreased quercetin aglycone recovery from the shallot digestate (p < 0.05), but had no significant effect on quercetin-3-glucoside recovery (p > 0.05). Digestion increased the Caco-2 cell uptake of shallot quercetin-4'-glucoside by 2-fold when compared to the non-digested shallot. Despite the loss of quercetin from the digested shallot, the bioavailability of quercetin aglycone to the Caco-2 cells was the same in both the digested and non- digested shallot. Treatment with lactase increased quercetin recovery from the shallot digestate nearly 10-fold and decreased quercetin-4'-glucoside recovery by more than 100-fold (p < 0.05), but had no effect on quercetin recovery from apple digestates. Lactase treatment also increased shallot quercetin bioavailability to the Caco-2 cells approximately 14-

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