Affordable Access

Publisher Website

Reconstituted Skin from Murine Embryonic Stem Cells

Authors
Journal
Current Biology
0960-9822
Publisher
Elsevier
Publication Date
Volume
13
Issue
10
Identifiers
DOI: 10.1016/s0960-9822(03)00296-3
Disciplines
  • Biology
  • Chemistry
  • Engineering

Abstract

Abstract Embryonic stem (ES) cell lines can be expanded indefinitely in culture while maintaining their potential to differentiate into any cell type [1, 2]. During embryonic development, the skin forms as a result of reciprocal interactions between mesoderm and ectoderm [3]. Here, we report the in vitro differentiation and enrichment of keratinocytes from murine ES cells seeded on extracellular matrix (ECM) in the presence of Bone Morphogenic Protein-4 (BMP-4) or ascorbate. The enriched preparation of keratinocytes was able to form an epidermal equivalent composed of a stratified epithelium when cultured at the air-liquid interface on a collagen-coated acellular substratum. Interestingly, an underlying cellular compartment that belongs to the fibroblast lineage was systematically formed between the reconstituted epidermis and the inert membrane. The resulting tissue displayed morphological patterns similar to normal embryonic skin, as evidenced by light and transmission electron microscopy. Immunohistochemical studies revealed expression patterns of cytokeratins, basement membrane (BM) proteins and late differentiation markers of epidermis, as well as fibroblast markers, similar to native skin. The results demonstrate the capacity of ES cells to reconstitute in vitro a fully differentiated skin. This ES-derived bioengineered skin provides a powerful tool for studying the molecular mechanisms controlling epidermal and dermal commitments.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

[Human skin from embryonic stem cells].

on Revue médicale suisse Dec 02, 2009

Murine embryonic stem cells.

on G Protein Coupled Receptors -... 2006
More articles like this..