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Isolated microspore derived plant formation via embryogenesis inTriticum aestivumL.

Authors
Journal
Plant Science
0168-9452
Publisher
Elsevier
Publication Date
Volume
48
Issue
1
Identifiers
DOI: 10.1016/0168-9452(87)90069-0
Keywords
  • Triticum Aestivum
  • Microspore Culture
  • Embryogenesis
  • Haploids
Disciplines
  • Biology

Abstract

Abstract Embryogenesis in isolated microspores of wheat ( Triticum aestivum L.) leading to plant regeneration has been established on modified liquid N6 medium (supplemented with 2,4-D, casein hydrolysate and Ficoll). Globular embryoids which were obtained after 6–8 weeks of culture of competent embryogenic microspores produced perfect embryoids when transferred to regeneration medium. Embryoids were differentiated to plants on other modified N6 agar medium (0.75% w/v agar, 20 g/l sucrose, 1 g/l myo-inositol, 8.8 μM 6-benzylaminopurine (BAP), 11.4 μM indoleacetic acid (IAA), 160 mg/l glutamine, 10 mg/l proline). Responses of microspores in regeneration and embryoid differentiation varied depending on the constituents of the media and genotypes used.

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