Affordable Access

Publisher Website

The Hep G2 cell line in the study of growth hormone receptor/ binding protein

Authors
Journal
Molecular and Cellular Endocrinology
0303-7207
Publisher
Elsevier
Publication Date
Volume
101
Identifiers
DOI: 10.1016/0303-7207(94)90216-x
Keywords
  • Growth Hormone
  • Growth Hormone Receptor
  • Hep G2 Cell
Disciplines
  • Biology

Abstract

Abstract This study identifies specific, high affinity GH-receptors (GH-R) in human hepatoma Hep G2 cells. The binding characteristics of GH-R in the Hep G2 cells are similar to those of human liver membranes, such as the high specificity for hGH, the binding affinity (K a = 1.7 ± 0.5 × 10 9 M −1) and the molecular weight of the membrane bound GH-R (apparent 125,000 and 71,000). In addition, lower molecular weight forms (∼ 94,000 and ∼ 58,000) were identified as GH-binding protein (GH-BP) in Hep G2 conditioned medium, or following incubation of Hep G2 cells, in the presence of 10 mM N-ethylmaleimide for 90 min at 30°C; the latter are presumed to be shed by a proteolytic cleavage of the GH-R. Exposure of Hep G2 cells to physiologic concentrations of hGH resulted in a concentration-dependent increase in 3H-thymidine incorporation, up to 48.4 ± 7.9% above control. In summary, the demonstration of specific, high affinity GH-R in Hep G2 cells, as well as shedding of GH-BP, suggest these cells may provide a homologous human system to study the receptor-effector interrelationship of hGH and to further our understanding of hepatocyte production of soluble GH-BP.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

The Hep G2 cell line in the study of growth hormon...

on Molecular and Cellular Endocri... May 1994

Insulin and variation in glucose levels modify the...

on Journal of Endocrinology December 1989

Inhibition of sex hormone-binding globulin product...

on The Journal of Clinical Endocr... September 1988
More articles like this..