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Effects of reactive oxygen species on arachidonic acid metabolism in rabbit platelets

Authors
Journal
Free Radical Biology and Medicine
0891-5849
Publisher
Elsevier
Publication Date
Volume
15
Issue
1
Identifiers
DOI: 10.1016/0891-5849(93)90129-i
Keywords
  • Reactive Oxygen Species
  • Cyclo-Oxygenase
  • Lipoxygenase
  • Rabbit Platelets
  • Free Radicals

Abstract

Abstract Rabbit platelets were exposed to a reactive oxygen species (ROS) generating system (xanthine plus xanthine oxidase) to explore the effect of ROS on the formation of 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE), thromboxane (TX) B 2, and 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT) from exogenous arachidonic acid. Xanthine plus xanthine oxidase suppressed the production of 12-HETE, TXB 2, and HHT by 65–69%. This effect was reversed by addition of catalase to the ROS generating system but not by superoxide dismutase, mannitol, or dimethylsulfoxide, indicating that H 2O 2 is the responsible metabolite. These results suggest that H 2O 2 plays an important role in the regulation of platelet cyclo-oxygenase and lipoxygenase activities.

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