Affordable Access

Publisher Website

Focal seizures disrupt protein synthesis in seizure pathways: An autoradiographic study using [1-14C]leucine

Authors
Journal
Brain Research
0006-8993
Publisher
Elsevier
Publication Date
Volume
248
Issue
1
Identifiers
DOI: 10.1016/0006-8993(82)91152-0
Keywords
  • Focal Seizures
  • Protein Synthesis
  • Seizure Pathways
  • Autoradiography
Disciplines
  • Biology

Abstract

Abstract We have used a new autoradiographic technique developed by Smith et al. 22,23 for visualizing rates of incorporation of [1- 14C]leucine into protein in brain. Focal seizures caused by topical convulsants resulted in a marked decrease in autoradiographic density. This was primarily confined to the seizure focus, especially marked in pyramidal cell layers, and to subcortical seizure pathways. There were no distinct changes in cortico-cortical pathways beyond the seizure focus. Pure orthodromic pathways through basal ganglia showed an 18% inhibition of leucine incorporation in caudate nucleus and substantia nigra, pars compacta ( P < 0.05). By contrast, thalamic nuclei connected both ortho- and antidromically to the focus showed a 30–63% inhibition ( P < 0.01). The topographic pattern and intensity of the thalamic changes were related to the site, size and intensity of the seizure focus As seizures became severe there was a more generalized depression of metabolism beyond seizure pathways, especially in the ipsilateral hemisphere. The results suggest that seizures block incorporation of leucine into protein either by an increase oxidation of the precursor, and/or an inhibition of protein synthesis per se. The effect is most severe in neurons undergoing epileptic burst discharge in the focus and in thalamic neuronal beds connected reciprocally with the focus.

There are no comments yet on this publication. Be the first to share your thoughts.