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Effects of tumour acidification with glucose+MIBG on the spontaneous metastatic potential of two murine cell lines

British Journal of Cancer
Nature Publishing Group
Publication Date
DOI: 10.1038/sj.bjc.6601766
  • Experimental Therapeutics
  • Ecology
  • Geography


Effects of tumour acidification with glucoseþMIBG on the spontaneous metastatic potential of two murine cell lines T Kallioma¨ki1,2 and RP Hill*,1,2,3 1Experimental Therapeutics Division, Ontario Cancer Institute/Princess Margaret Hospital, Toronto, Ontario, Canada M5G 2M9; 2Department of Medical Biophysics; 3Department of Radiation Oncology, University of Toronto, 610 University Avenue, Toronto, Ontario, Canada M5G 2M9 In addition to hypoxia, acidic extracellular pH (pHe) is recognised as one of the microenvironmental characteristics of solid tumours. A number of studies have examined ways to increase tumour acidity in order to improve tumour-specific targeting of certain drugs and the effectiveness of hyperthermia. However, previous data have shown that exposure of murine tumour cells to acid conditions in culture can enhance their metastatic potential when injected subsequently into mice, raising the concern that deliberate tumour acidification might increase the probability of metastasis. In this study, we examined the effects of in vivo tumour acidification and hypoxia on the spontaneous metastatic potential of the murine KHT-C fibrosarcoma and B16F1 melanoma cell lines. A tumour- specific increase in extracellular acidity, demonstrated by measurements with pH electrodes, was achieved by daily intraperitoneal injections of meta-iodo-benzylguanidine (MIBG) and/or glucose. This method of tumour acidification during tumour growth did not significantly enhance the spontaneous metastatic potential of the two murine cell lines. British Journal of Cancer (2004) 90, 1842–1849. doi:10.1038/sj.bjc.6601766 Published online 13 April 2004 & 2004 Cancer Research UK Keywords: acidic pH; MIBG; hypoxia; metastasis; murine tumours �� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � Tumour acidity is probably caused by excessive production of lactic and carbonic acids and their insufficient clearance by abnormal tumour vasculature (Newell et al, 199

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