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Problems in low-temperature spectrophotometry of turbid specimens

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
15
Issue
3
Identifiers
DOI: 10.1016/0003-2697(66)90107-2

Abstract

Abstract Deparession of the Soret absorption peak of reduced cytochrome c is partially caused by multiple reflection of forward scattered light, causing it to reach the photomultiplier without passing through the specimen. A simple insert for the Dewar which reduces multiple reflection of forward seattered light is described. A change in the ratio of Soret to α absorbance of reduced cytochrome c is observed on cooling to −196°C.

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