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Structural analysis by circular dichroism of some enzymes involved in plant cell wall degradation

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
282
Issue
2
Identifiers
DOI: 10.1016/0014-5793(91)80512-2
Keywords
  • Cellulase
  • Polygalacturonase
  • Xylanase
Disciplines
  • Biology

Abstract

Abstract Three enzymes which degrade different polysaccharide components of plant cell walls have been characterized by circular dichroism (CD). A bacterial endoglucanase, which in the native state forms part of a multiprotein cellulase complex, showed a tendency to form aggregates as measured by CD. Depending on its degree of aggregation, this enzyme displayed between 50% and 100% helical structure, whereas a bacterial xylanase and a fungal polygalacturonase exhibited more β-sheet structure. The polygalacturonase was apparently devoid of helical structure.

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