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Oxidized phospatidylcholine but not native phosphatidylcholine inhibits inducible nitric oxide synthase in RAW 264.7 macrophages

Authors
Journal
Life Sciences
0024-3205
Publisher
Elsevier
Publication Date
Volume
78
Issue
14
Identifiers
DOI: 10.1016/j.lfs.2005.07.035
Keywords
  • Oxidized Phospholipids
  • Atherosclerosis
  • Inducible Nitric Oxide Synthase
  • Murine Macrophages
Disciplines
  • Biology
  • Design

Abstract

Abstract This study was designed to compare the effects of oxidized 1-palmitoyl-2-arachidonyl- sn-glycero-3-phosphorylcholine (PAPC) and native PAPC on the inducible nitric oxide synthase (iNOS) in the macrophage cell line RAW 264.7. Macrophages stimulated by bacterial lipopolysaccharide (1 μg/ml) were incubated with increasing amounts of native or oxidized PAPC (oxPAPC, 10–20 μg/ml). Cells incubated with oxPAPC showed a dose-dependent inhibition of inducible nitric oxide synthesis, as well as reduced iNOS protein expression and mRNA levels. Additionally, chromatin immunoprecipitation assay revealed that oxPAPC reduced the interaction of the active NF-κB subunit p65 with the iNOS promoter region when compared to native PAPC.

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