Abstract The present study aimed at investigating mechanisms of selective uptake of HDL 3-associated cholesterylesters (HDL 3-CEs) by differentiated Ob1771 adipocytes. Our findings indicate that Ob1771 cells are capable of pronounced selective uptake of HDL 3-CEs in 2.6-fold excess of HDL 3 holoparticle uptake. Selective uptake of HDL 3-CEs into a releasable pool (presumably located in the cellular plasma membrane) was temperature insensitive while prominent internalization into a non-releasable and subsequent hydrolysis in a non-chloroquine sensitive compartment occurred at 37°C. Release of membrane bound endogenous LPL by heparin resulted in decreased HDL 3 holoparticle, total CE and selective CE uptake. Accordingly, the addition of exogenous LPL to the culture medium resulted in increased holoparticle, total CE and selective CE uptake.