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Selective uptake of high density lipoprotein-associated cholesterylesters by differentiated Ob1771 adipocytes is modulated by endogenous and exogenous lipoprotein lipase

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
414
Issue
3
Identifiers
DOI: 10.1016/s0014-5793(97)01061-2
Keywords
  • Selective Uptake
  • Adipocyte
  • Hdl3
  • Cholesterol
  • Lipoprotein
  • Lpl

Abstract

Abstract The present study aimed at investigating mechanisms of selective uptake of HDL 3-associated cholesterylesters (HDL 3-CEs) by differentiated Ob1771 adipocytes. Our findings indicate that Ob1771 cells are capable of pronounced selective uptake of HDL 3-CEs in 2.6-fold excess of HDL 3 holoparticle uptake. Selective uptake of HDL 3-CEs into a releasable pool (presumably located in the cellular plasma membrane) was temperature insensitive while prominent internalization into a non-releasable and subsequent hydrolysis in a non-chloroquine sensitive compartment occurred at 37°C. Release of membrane bound endogenous LPL by heparin resulted in decreased HDL 3 holoparticle, total CE and selective CE uptake. Accordingly, the addition of exogenous LPL to the culture medium resulted in increased holoparticle, total CE and selective CE uptake.

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