Affordable Access

Sequential release of BMP-7 and VEGF from the PLGA/AK-Gelatin Composite Scaffolds

Authors
Publisher
Trans Tech Publications Ltd.
Publication Date
Keywords
  • 060100 Biochemistry And Cell Biology
  • 090301 Biomaterials
  • Microspheres
  • Composite Scaffold
  • Growth Factor
  • Osteogenic Differentiation
  • Sequential Release
Disciplines
  • Biology

Abstract

Vascular endothelial growth factor (VEGF) and bone morphogenetic proteins (BMP-7) are key regulators of angiogenesis and osteogenesis during bone regeneration. The aim of this study was to investigate the possibility of realizing sequential release of the two growth factors using a novel composite scaffold. Poly(lactic-co-glycolic acid) (PLGA)-Akermanite (AK) microspheres were used to make the composite scaffold, which was then loaded with BMP-7, followed by embedding in a gelatin hydrogel matrix loaded with VEGF. The release profiles of the growth factors were studied and selected osteogenic related markers of bone marrow stromal cells (BMSCs) were analysed. It was shown that the composite scaffolds exhibited a fast initial burst release of VEGF within the first 3 days and a sustained slow release of BMP-7 over the full period of 20 days. The in vitro proliferation and differentiation of the BMSCs cultured in the osteogenic medium were enhanced by 1 to 2 times, resulting from the additionally and sequentially release of growth factors from the PLGA-AK/gelatin composite scaffolds.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Prolonged release from PLGA/HAp scaffolds containi...

on Journal of Materials Science M... February 2012

Optimized bone regeneration based on sustained rel...

on Biotechnology and bioengineeri... Mar 01, 2008

Effects of sequentially released BMP-2 and BMP-7 f...

on American journal of translatio... 2015
More articles like this..