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Structure of the human and murine R-rasgenes, novel genes closely related torasproto-oncogenes

Authors
Journal
Cell
0092-8674
Publisher
Elsevier
Publication Date
Volume
48
Issue
1
Identifiers
DOI: 10.1016/0092-8674(87)90364-3
Disciplines
  • Biology

Abstract

Abstract The human R- ras gene was isolated by low-stringency hybridization with a v-H- ras probe. The predicted 218 amino acid R- ras protein has an amino-terminal extension of 26 residues compared with H- ras p21, and shows 55% amino acid identity; conserved domains include the p21 GTP-binding site and the carboxy-terminal membrane localization sequence. R- ras has at least six exons, with the position of the first intron conserved relative to the Drosophila ras64B and Dictyostelium ras genes; there is no similarity in the exon-intron structure of the R- ras gene and of the mammalian H-, K-, and N- ras proto-oncogenes. Cloned mouse R- ras cDNAs exhibit 88% nucleotide and 94.5% predicted amino acid identity to human R- ras. Human R- ras was localized to chromosome 19, a site different from ras p21 genes. Mouse R- ras is syntenic with c-H- ras on chromosome 7.

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