Affordable Access

Publisher Website

A novel immunosensor forEnterobacter sakazakiibased on multiwalled carbon nanotube/ionic liquid/thionine modified electrode

Authors
Journal
Electrochimica Acta
0013-4686
Publisher
Elsevier
Publication Date
Volume
61
Identifiers
DOI: 10.1016/j.electacta.2011.11.092
Keywords
  • Enterobacter Sakazakii
  • Immunosensor
  • 1-Butyl-3-Methylimida-Zolium Hexafluorophosphate
  • Multiwalled Carbon Nanotube
  • Thionine
Disciplines
  • Chemistry

Abstract

Abstract A novel immunosensor for Enterobacter sakazakii ( E. sakazakii) based on screen-printed carbon electrode (SPCE) modified by multiwalled carbon nanotube (MWCNT), 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM]PF 6) and thionine (Thi) was developed for the first time. The surface morphologies of modified electrodes were characterized by means of atomic force microscope (AFM). The electrochemical performance of the immunosensor was evaluated by cyclic voltammetry. Horseradish peroxidase labeled antibody to E. sakazakii in [BMIM]PF 6 showed high activity and stability. Under optimal conditions, E. sakazakii could be detected in range of 10 3–10 9 CFU/ml with detection limit of 7.7 × 10 1 CFU/ml. The proposed immunosensor exhibited excellent long-time storage stability and had potential use in clinical immunoassay of E. sakazakii.

There are no comments yet on this publication. Be the first to share your thoughts.