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Corticotropin releasing factor stimulates adrenocorticotropin and β-endorphin release from AtT-20 mouse pituitary tumor cells

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
106
Issue
4
Identifiers
DOI: 10.1016/0006-291x(82)91264-5
Disciplines
  • Chemistry
  • Medicine

Abstract

Abstract Corticotropin releasing factor (CRF) was tested for its ability to stimulate ACTH and β-endorphin secretion from clonal AtT-20 D16-16 mouse pituitary tumor cells. Release of both hormones was stimulated 4 to 5-fold over the basal release at nanomolar concentrations of synthetic CRF. CRF analogues stimulated ACTH β-endorphin release with the same order of potency in the tumor cells as in primary cultures of anterior pituitary cells. A 90-min exposure to CRF elicited a 29–35% increase in total ACTH and β-endorphin immunoreactivity in tumor cell cultures. Dexamethasone markedly inhibited CRF-stimulated and basal ACTH and β-endorphin release. AtT-20 D16-16 cells may serve as a good model system for studying the biochemistry of CRF receptor-mediated events involved in ACTH β-endorphin release and synthesis.

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